Journal: Glia

Article Title: Astrocytic EAAT ‐2 Upregulation Supports Neuro‐Axonal Homeostasis in Chronic Inflammatory CNS Lesions

doi: 10.1002/glia.70243

Figure Lengend Snippet: Antibodies for immunofluorescence staining of mouse spinal cord tissue and human tissue.

Article Snippet: CD45 , PE , 30F11 , Miltenyi Biotec , 130‐117‐498 , Rat , ms , 1:20.

Techniques: Immunofluorescence, Staining, Tissue

Journal: Advanced Biology

Article Title: Fibro‐Adipogenic Progenitors From Skeletal Muscle Enhance Microvascular Angiogenesis in a 3D Model

doi: 10.1002/adbi.70162

Figure Lengend Snippet: Experimental Design and Characterization of FAPs and Freshly Isolated MVFs. (A) Schematic representing the study design (Created with BioRender.com). (B) Representative phase contrast image of a single microvascular fragment immediately after isolation. (C) Representative flow cytometry gating strategy and scatterplots of PDGFRα and SCA‐1 expression in FAPs after magnetic‐activated cell sorting.

Article Snippet: Briefly, the adherent cells were resuspended in 200 μL of DMEM + 2% FBS and incubated with antibodies (1 μL per 2.0 × 10 6 cells) against PDGFRα (ab203491, Abcam), SCA‐1 (AB4336, Millipore Sigma), CD31‐PE (130‐116‐538, Miltenyi Biotec), and CD45‐PE (130‐126‐312, Miltenyi Biotec) on ice for 30 min. For CD31/CD45 depletion, cells were incubated with 10 μL of Anti‐PE MicroBeads (130‐048‐801, Miltenyi Biotec) on ice for 30 min, washed twice in 1 mL of MACS buffer + 0.5% bovine serum albumin (BSA) via centrifugation (2000 rpm × 3 min at 4°C), and subjected to negative selection with LD Columns (130‐042‐901, Miltenyi Biotec) according to manufacturer protocols.

Techniques: Isolation, Phase Contrast, Flow Cytometry, Expressing, FACS, Co-Cultures, Confocal Microscopy